When light is passed through a coloured solution, certain wavelengths are selectively absorbed giving a plot of the absorption spectrum of the compound in solution. The wavelength at which maximum absorption is called the absorption maximum (λmax) of that compound. The light that is not absorbed is transmitted through the solution and gives the solution its colour.
Photometric instruments measure transmittance, which is defined as follows:
Intensity of the emergent (or transmitted) light Ie
Transmittance (T)= ---------------------------------------------------------------- = ------
Intensity of the incident light Io
Transmittance is usually expressed on a range of 0 to 100%.
Read more at : http://edusanjalbiochemist.blogspot.com/2015/12/photometry-principle-applications-and.html
Colorimetry priniciple |
No comments:
Post a Comment